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Cells or spores at a given temperature is called the decimal reduction time usually referred to as the D-value Larousse and Brown 1997. The D-value usually varies inversely with temperature.

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However FISH has also been developed to target RNA and thus visualize gene expression in situ herein referred to as RNA-FISH Singer Ward 1982.

. More recently computational and imaging technology has further driven the development of RNA-FISH to allow for the visualization and semi-automated quantification of individual mRNA transcripts.

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